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The helicases DinG, Rep and UvrD cooperate to promote replication across transcription units in vivo

Tipo: Artículo de revista
Referencia Autores: Boubakri H. Langlois A., Viguera E., Michel, B
Autores pertenecientes a este Departamento: Enrique Viguera Mínguez
Revista: EMBO Journal
Año de publicación: 2010
Volumen: 29
Páginas: 145-157
Artículo: http://www.nature.com/emboj/journal/v29/n1/full/emboj2009390a.html
Abstract:
How living cells deal with head-on collisions of the replication
and transcription complexes has been debated for a
long time. Even in the widely studied model bacteria
Escherichia coli, the enzymes that take care of such
collisions are still unknown. We report here that in vivo,
the DinG, Rep and UvrD helicases are essential for efficient
replication across highly transcribed regions. We show that
when rRNA operons (rrn) are inverted to face replication,
the viability of the dinG mutant is affected and over-expression
of RNase H rescues the growth defect, showing that
DinG acts in vivo to remove R-loops. In addition, DinG, Rep
and UvrD exert a common function, which requires the
presence of two of these three helicases. After replication
blockage by an inverted rrn, Rep in conjunction with DinG
or UvrD removes RNA polymerase, a task that is fulfilled in
its absence by the SOS-induced DinG and UvrD helicases.
Finally, Rep and UvrD also act at inverted sequences
other than rrn, and promote replication through highly
transcribed regions in wild-type E. coli.

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