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Identification of novel virulence genes and metabolic pathways required for full fitness of Pseudomonas savastanoi pv. savastanoi in olive knots

Tipo: Artículo de revista
Referencia Autores: I.M. Matas, L. Lambertsen, L. Rodríguez-Moreno, C. Ramos
Autores pertenecientes a este Departamento: Cayo Ramos Rodríguez
Revista: New Phytologist
Año de publicación: 2012
Volumen: 196
Páginas: 1182-1196
ISSN: doi: 10.1111/j.1469-8137.2012.
Artículo: [descargar archivo]
Abstract:
Comparative genomics and functional analysis of Pseudomonas syringae and related pathogens have mainly focused on diseases of herbaceous plants; however, there is a general lack of knowledge on the virulence and pathogenicity determinants required for infection of woody plants.
Here we apply signature-tagged mutagenesis (STM) to Pseudomonas savastanoi pv. savastanoi during colonization of olive knots, with the goal of identifying the range of genes linked to growth and symptom production in its plant host.
A total of 58 different genes were identified and most mutations resulted in hypovirulence in woody olive plants. Sequence analysis of STM mutations allowed us to identify metabolic pathways required for full fitness of P. savastanoi in olive and revealed novel mechanisms involved in the virulence of this pathogen, some of which are essential for full colonization of olive knots by the pathogen and for the lysis of host cells.
This first application of STM to a P. syringae-like pathogen provides confirmation for functional capabilities long believed to play a role in survival and virulence of these group of pathogens but not adequately tested before, and unravels novel factors not correlated previously with the virulence of other plant or animal bacterial pathogens.


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